By Curtis A. Machida
Oregon healthiness Sciences Univ., Beaverton. offers a set of reproducible tools for the research of regulators of CNS-mediated habit and neural functionality. For neurobiologists, neurochemists, neurologists, and pharmacologists. DNLM: Receptors, Adrenergic - research.
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313–324. Receptor Gene Isolation 51 10. Mack, A. (1996) Kits and other new developments streamline DNA purification process. html. 11. Brush, M. (1997) Cycle sequencing kits. The Scientist 11, 16–18, http:www. html. 12. Cheng, S. (1995) Longer PCR amplifications, in PCR Strategies (Innis, M. , Gelfand, D. , and Sninsky, J. ), Academic, San Diego, CA, pp. 313–324. 13. Preston, G. (1996) Polymerase chain reaction with degenerate oligonucleotide primers to clone gene family members, in Methods in Molecular Biology, vol.
And Maniatis, T. , Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. 2. Yu, L. and Bloem, J. J. (1996) Use of polymerase chain reaction to screen phage libraries in Methods in Molecular Biology, vol. 58: Basic DNA and RNA Protocols, (Harwood, A. ), Humana, Totowa, NJ, pp. 335–339. 3. Takumi, T. and Lodish, H. F. (1994) Rapid cDNA cloning by PCR screening. Biotechniques 17, 443–444. 4. , Kingston, R. , Moore, D. , Seidman, J. , Smith, J. , et al. (1995) Mapping by multiple endonuclease digestions, in Current Protocols in Molecular Biology, vol.
Oligonucleotides can range from 18 to 40 nucleotides in length, but for most applications 18–24 bp are sufficient. 4. The sense and antisense primers should have approximately the same G + C content (40–60%). The melting temperatures, Tms, for each primer should be within 1–2°C of each other (see Note 12). 5. The primer annealing temperature for PCR is approx 5°C lower than the Tm of the oligonucleotides (see Note 13). 6. The selection of primers from known sequences can be determined visually or with computer programs.
Adrenergic Receptor Protocols by Curtis A. Machida